雷帕霉素对人肝癌裸鼠原位移植瘤生长的影响

Effect of rapamycin on the growth of hepatocellular carcinoma in nude mice

目的探讨免疫抑制剂雷帕霉素对肝癌细胞生物学行为的影响。方法建立肝癌裸鼠原位移植瘤模型80只,随机分为空白对照组、环孢霉素(CsA)处理组、雷帕霉素常规剂量处理组和高剂量处理组,每组20只。用药2周后观察肿瘤生长情况,RT-PCR方法检测肿瘤组织中CD44v6 mRNA的表达。结果雷帕霉素常规剂量组及高剂量组肿瘤体积较空白对照组明显缩小,CD44v6的表达显著下调(P<0.05);CsA组肿瘤体积较之空白对照组明显增大,CD44v6的表达显著上调(P<0.05)。结论CsA可促进肝癌的生长和转移;雷帕霉素具有显著抑制肝癌侵袭转移的作用,能抑制肝癌细胞CD44v6基因的表达。

Objective To study the effect of rapamycin on the biological characteristics of human hepatocellular carcinoma （HCC）. Methods Eighty nude mice bearing orthotopic human HCC were divided randomizely into 4 groups： control group, cyclosporine （CsA） treatment group, and rapamycin （routine dose） treatment group, and rapamycin （high dose ） treatment group. Two weeks after treatment, the influence of rapamycin on the growth of hepatocellular carcinoma was observed, and RT-PCR was used to detect CD44v6 mRNA expression in the hepatocellular carcinoma tissues. Results As compare with control group, the tumor volume significantly decreased in routine dose rapamycin group and high dose rapamycin group, but increased in CsA group, The expression of CD44v6 mRNA was downregulated in routine dose rapamycin group and high dose rapamycin group, but upregulated in CsA group, as compared with control group. Conclusions CsA can promote the growth and metastasis of hepatocellular carcinoma cells. Rapamycin can inhibit the growth and invasiveness of hepatocellular carcinoma cells. Rapamycin also can inhibit CD44v6 gene expression.