柯萨奇B4病毒jlu06株基因序列及遗传进化分析

Sequence and Phylogenetic Analysis of jlu06 Strain of Coxsackievirus B4

目的对柯萨奇B4病毒(CVB4)jlu06株进行基因序列及遗传进化分析,并探讨其与致病相关的分子基础。方法Trizol法提取病毒RNA,RT-PCR扩增目的基因片段,测定基因组全序列,并进行序列同源性及遗传进化分析。结果CVB4 jlu06株基因组全长7 395个核苷酸,编码2 183个氨基酸,与其他CVB4株的同源性较高,且发生了10个氨基酸的变异,与HumanCB4和POLG_CXB4亲缘关系较近,与CXB2亲缘关系较远;CVB4 jlu06株与具有潜在致糖尿病性病毒株,在非结构蛋白3A区4个位点有共同的氨基酸,与非致病毒株不同。结论CVB4 jlu06株具有典型的CVB4基因组特征,在非结构蛋白3A区发生的核苷酸和氨基酸变异可能与其致病性相关。

Objective To analyze the gene sequence and phylogenesis of jlu06 strain of coxsackievirus B4 （CVB4） and investigate the pathogenicity-related molecular character of the strain. Methods The viral RNA of CVB4 was extracted by Trizol method and used as a template for amplification of target gene by RT-PCR. The whole genome of amplified product was sequenced and analyzed for homology and phylogenesis. Results The whole geuome of CVB4 jlu06 strain consisted of 7 395 nueleotides encoding 2 183 amino acids, which showed high homologies to other CVB4 strains, with the mutations of 10 amino acids. Phylogenetie analysis proved that CVB4 jlu06 strain showed close relationship to HumanCB4 and POLG_CXB4 strains while showed remote relationship to CXB2 strain. Unlike other non-diabetogenic strains, CVB4 jlu06 strain showed common amino acids with diahetogenic strains at 4 sites in 3A region of non-structural protein. Conclusion CVB4 jlu06 strain showed typical genomic character of CVB4. The mutations of nucleotides and amino acids at 3A region of non-structural protein might be related to the pathogenicity of CVB4 jlu06 strain.