人类白细胞抗原DQB1 ＊0602位点基因多态性与汉族人重度慢性牙周炎易感性关系的研究

Association of HLA DQB1 ＊ 0602 Polymorphism with the Susceptibility to Severe Chronic Periodontitis in Chinese Han Nationality

目的探讨人类白细胞抗原(human leucocyte antigen,HLA)-Ⅱ类基因区基因多态性与重度慢性牙周炎易感性的关系,为寻找汉族人群重度慢性牙周炎易感基因或抗病基因提供线索。方法选取重度慢性牙周炎患者134例,健康对照81人,取颊黏膜拭子,提取DNA,采用聚合酶链式反应-限制性内切酶片段长度多态性分析方检测HLA DQB1*0602多态性,比较两组DQB1*0602等位基因的频率分布。结果重度慢性牙周炎组与健康对照组HLA DQB1*0602等位基因频率分别为0.295、0.160,差异有统计学意义(χ2=9.864,P=0.002),相对危险度RR=1.84。结论HLA DQB1*0602位点基因多态性与汉族人群重度慢性牙周炎遗传易感性有一定关系。

Objective To investigate the association of HIM DQB1 ＊ 0602 polymorphism with the susceptibility to severe chronic periodontitis （CP） in Chinese Han nationality, so as to provide possible evidence for seeking the potential susceptibility gene or resistant gene of periodontitis in the subjected cohort. Methods DNA samples were collected with buccal swabs from 134 subjects with severe chronic periodontitis and 81 periodontal healthy controls. HIM DQB1 ＊ 0602 genotypes were determined using polymerase chain reaction-restrlction fragment length polymorphism （PCR-RFLP）. The genotype distribution was analyzed. Results The allele frequencies of DQB1 ＊ 0602 was detected more frequently in severe CP patients than in the controls （RR = 1.84, P 〈 0.05 ）. Conclusion The results suggest that HLA DQB1 ＊ 0602 genotype may be a risk indicator for the susceptibility to severe chronic periodontitis in Chinese Han nationality.