利胆片中药材的鉴别及大黄的含量测定

Identifications of Lidan Tablets and determination of Rheum officinale Baill

目的:提高利胆片的质量标准。方法:采用薄层色谱(TLC)法鉴别金银花、黄芩、知母及白芍,用高效液相色谱(HPLC)法测定方中大黄的含量。结果:TLC鉴别斑点明显。含量测定大黄素0.01064～0.3192μg、大黄酚0.02586～0.7758μg,进样量与峰面积线性关系良好。平均加样回收率大黄素为99.80%,RSD=0.78%(n=6);大黄酚为99.72%,RSD=1.18%(n=6)。结论:建立的TLC鉴别和HPLC含量测定方法专属性强、重复性好,可用于利胆片的质量控制。

Objective：To improve the quality standard of Lidan Tablets. Methods：TLC and HPLC were used.Results：The TLC method could indentify Flos Lonieerae,Scutellaria,Timosaponin and Paeony.The HPLC method could quantitatively determine the total content of emodin and chrysaphanol in Lidan Tablets.The linear range of emodin was 0.01064-0.3192 μg （r=0.9999） and the average re-covery was 99.8% with RSD of 0.78% ; the linear range of chrysaphanol was 0.02586-0.7758 μg （r=0.9999） and the average recovery was 99.7% with RSD of 1.18%.Conclusion：The indentification methods are reproducible and specific.The quantitative determination method is simple and accurate.