吸入麻醉剂浓度及作用时间对人离体精子活率的影响

Effects of Inhalation Anesthetics Concentration and Exposure Time on Human Sperm Motility in Vitro

目的：研究吸入麻醉剂浓度及作用时间对人离体精子活率的影响。方法：选择人精液20份，上游法优化处理后随机分为异氟醚实验组和七氟醚实验组，各10份。分别观测5个时间点（0．5h、1h、2h、3h、4h）及5个浓度水平（0、1．4％、2．8％、4．2％、5．6％）异氟醚和相似浓度七氟醚对精子活率的影响，精子运动功能采用计算机辅助精子分析系统分析。结果：1．4％～5．6％异氟醚作用于精子0．5～4h后，精子活率显著升高，其变化趋势符合S型曲线，相同浓度异氟醚组，精子活率随放置时间的延长成直线下降。类似浓度七氟醚对精子活率无显著影响。结论：临床浓度异氟醚作用于人离体精子，可以显著升高精子活率。类似浓度七氟醚对精子活率无显著影响。

Objective： To study the effects of inhalation anesthetics （isoflurane and sevoflurane） concentration and exposure time on human sperm motility in vitro. Methods： The sperm samples were random obtained from 20 healthy men by masturbation and prepared by swim-up technique. Using computer-assisted sperm analysis （CASA）, the effects of isoflurane and sevoflurane at clinical concentration （1.4%-5.6%） and exposure time （0.5-4h） on human sperm motility in vitro were observed at 25℃. Results： Isoflurane significantly increased the apparent motility of human at the 1.4%-5.6% concentration with the exposure time for 0.5-4h and the change trend was to correspond with S curve. When the concentration of isoflurane was excluded, sperm motility declined linear by exposure time. Sevoflurane had little effect on human sperm motility at the same concentration. Conclusion： Isoflurane can increase human sperm motility at clinical concentration and sevoflurane had little effect on human sperm motility.