心复宁V号对垂体后叶素致急性心肌缺血大鼠血浆TXA2及PG12的影响

Effect of Xinfuning No.V on TXA2&PG12 in Plasma of Myocardial Ischemia Rats with Pituitrin

目的:观察心复宁V号对垂体后叶素所致急性心肌缺血大鼠血浆TXA2及PGI2的影响。方法:60只大鼠,随机分成6组,即空白对照组、模型对照组、心复宁V号大剂量组(17.08g/kg)、心复宁V号中剂量组(8.54 g/kg)、心复宁V号小剂量组(4.27g/kg)、阳性对照组(单硝酸异山梨酯)。采用灌胃给药,每天1次,连续14d。末次给药后1h,舌下静脉给予垂体后叶素造成急性心肌缺血模型。1h后取血,分离血浆,测定各组TXA2、PGI2的代谢产物6-Keto-PGF1a、TXB2,分析PGI2/TXA2比值。结果:大鼠给予垂体后叶素后,血浆中TXB2升高,6-Keto-PGF1a/TXB2比值降低(P<0.05、0.01)。与模型对照组比较,心复宁V号(17.08g/kg,8.54 g/kg)明显降低垂体后叶素所升高的TXB2,升高6-Keto-PGF1a/TXB2(P<0.01)。结论:心复宁V号抗垂体后叶素致大鼠心肌缺血作用机制之一是通过抑制升高的血浆TXA2,纠正PGI2/TXA2失衡。

Objective .To observe the effect of XFN-V on TXA2 and PG12 in blood plasma of acute myocardial ischemia rats caused by injcoting pituitrin. Methods. 60 Wistar rats were divided into 6 groups at randora：normal control group, model control group, high dosage XFN-V group （17. 08g/ kg）, middle dosage XFN-V group（8. 54 g/kg）,low dosage XFN-V group（4. 27g/kg）, positive control group （ISMN）. Intragastric adminis-tration was adopted once a day for 14 days. Acute ischemia model rat was established by injecting pituitrin from sublingual vein lh after the last administration, and blood plasma was separated to be determined the activity of TXB2 and 6-Keto-PGFla that is the metabolite of TXA2 and PGlz in blood plasma. Results ： Having been injected pituitrin,the activity of TXB2 upgraded while 6-Keto-PGF1a/XB2 decreased（P（0.05, 0. 01）. Compared with model group, XFN-V（17.08g/ kg, 8. 54 g/kg）group could decrease the activity of TXB2 and increase the ratio of 6-Keto-PGF1a/TXB2 （P〈0.01）. Conclusion .One of the action mechanisms of XFN-V ameliorating myocardial ischemia caused by pituitrin is that it can increase the ratio of 6-Keto-PGF1a/TXP2 through decreasing the activity of TXA2.