液相色谱法测定环氧乙烷和乙二醇装置中气体和液体的醛含量

Determination of formaldehyde and acetaldehyde content in the gas and liquid sample in the ethylene oxide and EG equipment with HPLC

衍生技术与液相色谱法相结合,将样品与过量的2,4-二硝基苯肼衍生液混合,样品中的甲醛、乙醛分别与2,4-二硝基苯肼发生衍生反应,生成甲醛、乙醛的腙衍生物,用高效液相色谱ODS柱分离,采用DAD检测器在254nm波长下分别测量峰面积,应用标准曲线外标方法计算结果,准确定量测定样品中的甲醛、乙醛含量,解决了环氧乙烷装置反应器出口气中、乙二醇水溶液中以及环氧乙烷和乙二醇产品中的甲醛、乙醛分别定量测定的技术难题,高效、灵敏、准确。

Derivation method was combined with HPLC, The sample was mixed with excess 2,4-dinitrophenyl- hydrazine derivative liquid. Derivative reaction was occured between formaldehyde, acetaldehyde in the sample and dinitrophenylhydrazine separately. The hydrazone derivatives of formaldehyde and acetaldehyde was produced, then separated with HPLC ODS column. The DAD detector was used to measure the peak area under the wavelength of 254 nm,the standard curve external standard method was used to calculate the results. The formadehyde and ac- etaldhyde content in the sample was determined aecurately. The technique problems that respectively quantitative determination of formaldehyde and acetaldehyde content in the outlet gas of ethylene oxide equipment reactor, in ethylene glycol solution and in ethylene oxide and ethylene glycol product were solved. The determination method is efficient, sensitive and accurate