子宫内膜癌中pRb2/p130的表达及意义

Expression of pRb2/p130 Gene in Human Endometrial Carcinoma Cells and the Significance

目的探讨子宫内膜组织中pRb2/p130的表达及其与子宫内膜癌临床病理特征的关系。方法应用免疫组化SP法检测pRb2/p130在28例正常增生期子宫内膜、20例不典型增生子宫内膜、30例子宫内膜腺癌组织中的表达。结果pRb2/p130阳性表达率在正常增生期子宫内膜、不典型增生子宫内膜、子宫内膜腺癌组织中依次递减(分别为82.1%、55.0%、23.3%),且子宫内膜腺癌和不典型性增生中明显低于正常增生期子宫内膜,差异均有统计学意义(P<0.05);pRb2/p130阳性表达缺失与组织分化程度相关(r=-0.446,P<0.05),与临床分期、子宫肌层浸润程度无相关性(P>0.05)。结论在子宫内膜癌中pRb2/p130的表达下降或缺失是一个频发事情,其恶性度愈高阳性率愈低,提示pRb2/p130与子宫内膜癌的发生、发展密切相关,并在其发病机制中起着重要的作用。

Objective To investigate the expression of pRb2/p130 gene and its relationship with the patholgical clinical features of human endometrial carcinoma. Methods The levels of pRb2/P130 protein were examined with immunohistochemical SP method in 28 staples of normal endometrial tissue,20 samples of atypical endometrial hyperplasia and 30 samples of endometrial adenocarcinomas. Results The positive rate of pRb2/p130 protein in the normal endometrium,atypical endometrial hyperplasia cells and endometrial adenocarcinoma cells was respectively 82.1 % ,55.0% and 23.3 %. pRb2/p130 protein in endometrial atypical hyperplasia or endometrial adenocarcinoma was significantly lower than that in the normal tissue （P〈0. 05）. The positive expression of pRb2/p130 gene was related to histological differentiation （r=-0.446 ,P〈0.05）, but it＇s not related to clinical stages and depth of invasion （P〉0.05）. Conclusion Descent or de- letion of the expression of pRb2/p130 gene is of a frequent incidence in human endometrial carcinoma. The positive rate of pRb2/p130 expression is reversly proportionate to malignant degree of endometrial carcinoma,which indicats that pRb2/p130 plays an important role in the development and pathogenesis of endometrial carcinoma.