HIV抗体检测替代策略Ⅲ在江西口岸的应用

Application of Alternative Strategy Ⅲ in HIV Antibody Test at Jiangxi Port

〔目的〕寻找用于江西口岸的HIV抗体检测筛查程序,及时准确出具HIV抗体检测报告。〔方法〕分析本中心初筛确认为HIV抗体阳性的28份标本,应用免疫印迹法(WB)进行确认的结果,再采用《全国艾滋病检测技术规范(2004年版)》第2章HIV抗体检测中的替代策略Ⅲ进行验证比较。〔结果〕ELISA和另一种不同原理的胶体硒筛查方法呈阳性反应的标本,用明胶颗粒凝集方法检测,结果均呈阳性反应,与确认结果一致;仅ELISA筛查出现阳性的标本,用胶体硒与明胶颗粒凝集方法检测结果为阴性,确认检测结果为阴性或不确定。〔结论〕应用HIV抗体检测的替代策略Ⅲ,可以为江西口岸HIV的筛查出具快速、准确的HIV抗体阴性报告提供技术上的依据。

Objective To find the screening procedures of HIV antibody test at Jiangxi Port, and to timely and accurately issue reports of HIV test. Method In this study, 28 cases as HIV antibody positive were analyzed and confirmed by WB assay. Meanwhile, the ＂alternative Strategies Ⅲ in HIV antibody test＂ in chapter 2 of ＂National HIV Testing Technology specification （edition 2004）＂ was adopted to verify and compare. Result The samples by using ELISA and Electroselenium method of different principle screening was positive. The results were positive by using PA method, and consisitent whith confirmed results. The samples were positive by ELISA, however the testing results were negative by Electroselenium and PA method, the results were confirmed as negative or uncertain. Conclusion The application of Alternative Strategies Ⅲ in HIV antibody test can provide a technical basis for issuing the negative report of HIV screening accurately and rapidly at Jiangxi Ports.