猪糖尿病模型的制作及评价

Establishment and evaluation of experimental diabetic models in pigs

目的:探索一种较好的类似于人类糖尿病的模型的方法。方法:选用贵州小香猪,联合应用链脲菌素、四氧嘧啶造模,连续监测血糖浓度;ELISA方法检测造模前后血清胰岛素水平。胰腺HE切片用来评价胰岛状态。结果:造模前,糖尿病模型组和正常对照组的体重、血糖和胰岛素水平均无显著性差异。造模后,与对照组相比,模型组体重显著下降(4.09±0.31 vs.6.42±0.56 kg,P<0.05),血糖显著升高(17.30±1.20 vs.4.30±0.32 mmol/L,P<0.05),胰岛素水平显著下降(0.04±0.02 vs.0.11±0.03μg/L,P<0.05)。HE切片显示模型组胰岛明显萎缩。结论:给小型猪静脉注射60 mg/kg链脲菌素和30 mg/kg四氧嘧啶可成功建立糖尿病模型,该剂量可作为复制猪糖尿病模型的参考标准。

Objective： To explore a better type 1 diabetes models similar to that of humans. Methods： Male Chinese laboratory miniature pigs administrated with streptozotocin and alloxan were used as subjects. The dynamic changes of the glucose contents in plasma were continuously measured and insulin was measured by ELISA. HE stained slides were used to value the islets state. Results：There were no significant difference in weight, glucose level and insulin level between diabetic group and controls before being models. Compared with normal controls： weight of models decreased （4. 09 ±0. 31 vs. 6. 42 ±0. 56 kg, P 〈0. 05） ; the glucose contents increased significantly（ 17. 30 ± 1.20 vs. 4. 30 ± 0. 32 mmol/L ,P 〈 0. 05 ）and insulin contents reduced （0. 04 ± 0. 02 vs. 0. 11 ±0. 03 ug/L, P 〈 0. 05 ）in plasma. HE stained slides showed atrophic islets in diabetic models. Conclusion： The porcine type 1 diabetes mellitus models has been established successfully by injection of Streptozotocin （60 mg/kg） and Alloxan （30 mg/kg）. The dose in this study could be eonsidered as a reference standard for porcine diabetic models.