荧光光度法测定自来水及尿样中痕量钼

Spectrofluorimetric determination of trace amounts of molybdenum in running water and urine

目的：自来水和尿样中痕量钼的测定。方法：合成了新荧光试剂2-羟基苯乙酮苯甲酰腙（HAPBH）,研究了试剂与钼的荧光反应条件。结果：在pH=4.0的溶液中,该试剂与钼形成稳定的配合物,配合物的激发和发射波长为λex/em=306/437 nm。结论：荧光强度与钼的浓度在0-120μg/L内成线性关系,检出限为0.057μg/L,方法成功的应用于自来水和尿样中痕量钼的测定。

Objective：To determine trace amounts of molybdenum in running water and urine. Methods：A fluorescent reagent 2 - hydroxyacetophenone benzoylhydrazone （HAPBH） was synthesized. The fluorescent reaction of this reagent with molybdenum was studied. Results：Molybdenum could form a stable complex with 2 - hydroxyacetophenone benzoylhydrazone （ HAPBH）, in the solution at pH 4.0. The Mo - HAPBH complex has excitation and emission maxima at 306 and 437 nm, respectively. Conclusion：The linear range of the method is from 0 to 120ug/L and the detection limit is 0. 057 ug/L of molybdenum. This method has been successfully applied to the determination of molybdenum in running water and urine.