PCR和传统方法在大样本低概率细菌检验项目中的联合应用

Combining PCR and traditional methods in bacterial detection with large sample and low probability

目的:建立提高大样本低概率的细菌阳性检出率,降低工作强度,节约检测成本的一种检测程序。方法:采用高度特异、敏感的PCR作为样品初筛试验,结合传统方法培养、分离、鉴定。结果:342份奶粉样品,PCR检测阳性16份,阳性率为4.68%;传统方法检测阳性样品5份,阳性率为1.46%。检测耗时折算PCR法仅为传统方法的16.67%(10/60)。结论:PCR作为初筛试验,与传统方法的联合应用,为致病菌检测提供了一个新的思路,值得在大样本低概率细菌检验项目中推广应用。

Objective：A method for detecting bacteria was established to increase positive rate,decrease working strength and reduce cost. methods：Combining highly specific and sensitive PCR with traditional cultivation, isolation and identification methods. Rusults：Among 342 powdered infant formula samples, 16 positive samples （4. 68% ） were detected by PCR, and 5 positive samples （ 1.46% ） by traditional method, suggesting that the positive rate by PCR was higher than that by traditional method. Time - consuming for PCR is only 16. 67 % of the traditional one. Conclusion：The combination of PCR with traditional method presents us a new way for food - borne pathogen detection.