影响用化学发光免疫法检测尿游离皮质醇的重要因素

Influencing factors of urine free cortisol measurement by chemiluminescence immunoassay

目的:探讨用化学发光免疫法检测尿游离皮质醇(UFC)的影响因素。方法:用化学发光免疫测定药盒对加入不同防腐剂的标本、存放温度不同的标本和24 h尿量不同的标本进行测定。结果:防腐剂为盐酸和叠氮钠时,UFC测定水平较低(P<0.001),置于4℃的标本的UFC略高于置于-20℃的标本,而未服强的松龙时的UFC水平则较服药时显著降低(P<0.001)。结论:防腐剂和24 h尿量对UFC测定水平均有不同程度的影响。

Objective：To investigate the factors that effected the measurement of urine free cortisol （UFC） by chemiluminescence immunoassay. Methods：Chemiluminescence immunoassay kit was used to test UFC level in the following samples： samples preserved by different preservative agents, samples preserved under various temperature and samples of various 24 hour urine volume. Results：UFC level was lower （P 〈 0. 001 ） when using hydrochloric acid and sodium azide as the preservative agent. Compared with - 20℃, UFC level of samples preserved under 4℃ slightly increased. UFC level of patients who were taking prednisolone significantly increased as compared with their baseline level without prednisolone （P 〈 0. 001 ）. Conclusion：Preservative agents and 24 hour urine volume have significant effect on the measurement of UFC level.