千年健超临界CO_2萃取工艺及萃取物包合工艺研究

Study on Extraction with Supercritical CO_2 Fluid from Rhizoma Homalomena and Its Inclusion Process

目的研究千年健超临界CO2萃取及萃取物包合的最佳工艺,并测定萃取物的化学成分。方法采用均匀设计方法,以萃取压力、萃取温度、萃取时间为因素,考察超临界CO2萃取的最佳工艺条件并对萃取物进行GC-MS分析;采用正交设计方法,以包合物收率,包合物油利用率、包合物含油率为指标,研究主客分子比、碾磨时间、加水倍量、加醇量对包合结果的影响并进行药效学研究。结果超临界CO2萃取工艺为:压力20MPa,温度60℃,时间30min;包合最佳工艺为主客分子比1∶4,研磨30min,加入2倍量的水,含醇量20%;萃取物包合物具有显著的抗炎、镇痛作用;所得萃取物主要成分为芳樟醇。结论利用超临界CO2萃取技术萃取千年健,可以缩短萃取时间,明显提高收率;优选出的包合工艺参数稳定,可行。

OBJECTIVE To study the optimum parameters of the supercritical CO2 fluid extraction of Rhizoma Homalomena and its inclusion, and to determine the chemical constituents. METHODS he experiment was performed with uniform design. The factors were extractive pressure, temperature, extractive time and the flow rate of CO2. By orthogonal design with the extracts of inclusion, the inclusion yield, the oil in inclusion complex and the utilization ratio ofoil, as index the four effect factors, including the proportion of oil to β-cyclodextrin, milling time, water addition and ethanol addition were evaluated. The pharmacodynamics was studied. RESULTS The optimum condition were obtained： the extractive pressure was 20 MPa, the temperature of extractive was 60 ℃, the extractive time was 30 min. The ratio ofoil to β-cyclodextrin was 1 ： 4, the milling time was 30 min. Two volumes of water was added, ethanol was 20%. Extracts from the inclusion showed significant anti-inflammatory and analgesic effect. The main chemical constituents was Linalool. CONCLUSION The method applied to obtain the extractive matters from Rhizoma Homalomena was quickly and efficiently with satisfactory results. The inclusion process is stability and feasible.