摘要
目的建立高效液相色谱法测定硫鸟嘌呤原料及片剂的含量及有关物质。方法采用AltimaC18色谱柱,以0.05mol·L-1磷酸二氢钠溶液(pH3.0)为流动相,柱温为35℃;检测波长248nm;流速1.0mL·min-1。结果硫鸟嘌呤在8～120mg·L-1内线性关系良好(r=0.9999),最低检测限为0.12mg·L-1,回收率为99.27%。结论本方法准确、灵敏,精密度高、专属性强,可用于硫鸟嘌呤含量及有关物质的测定。
OBJECTIVE To establish a reversed-phase high performance liquid chromatography method for the determination of the thioguanine and its related substances. METHODS The analytical column was Altima C18 and the mobile phase was 0.05 mol·L^-1 sodium dihydrogen phosphate acid （pH 3.0）. The column temperature was maintained at 35℃. The detection wavelength was 248 nm. The flow rate was 1.0 mL·min^-1. RESULTS Thioguanine was linear in the range from 8 to 120 mg·L^-1 （r=0.999 9）. The limit of detection was 0.12 mg·L^-1. The average recoveries were 99.27%. CONCLUSION The method was proved to be accurate, sensitive and precise and showed highly specific. It can be applied for the content assay, as well as the related substances of thioguanine
出处
《中国药学杂志》
CAS
CSCD
北大核心
2009年第13期1029-1032,共4页
Chinese Pharmaceutical Journal
基金
国家药典委员会2010版药典专项基金(国药典化发〔2008〕97号)
