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利用间接凝集方法检测唾液ABH血型物质的初步研究

Preliminary Study on Detecting A,B,H Substance in Saliva Through the Indirect Agglutination Reaction
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摘要 建立一种新的检测唾液中ABH血型物质(均属于唾液粘蛋白MG1)的方法。利用间接凝集反应通过黏附于含钙微粒上的唾液粘蛋白MG1与相应血型抗体结合而呈现明显的凝集现象,来检测唾液中相应血型物质的存在。以传统方法为金标准,本方法检测ABH物质的准确度分别为87.8%(72/82)、89.0%(73/82)和30.5%(25/82);灵敏度分别为64.3%(18/28)、71.9%(23/32)和9.5%(6/63);特异性均为100%。本实验初步建立了利用间接凝集反应检测唾液中A、B、H血型物质的不同于传统的新方法,对于唾液血型物质检测方法学的研究具有重要意义,同时为利用相应的特异性抗体定性检测唾液粘蛋白MG1提供了参考依据。 To develop a new method to test the existence of A, B and H substance ( all along to the salivary mucin MG1 ) in saliva. The blood group substances in saliva were detected by indirect agglutination reaction between salivary mucin MG1 which adhered to calcium-containing particle and the corresponding blood group antibody. The results showed that the accuracy of A, B and H substance compared with the traditional method were 87.8% (72/82) ,89.0% (73/82)and 30.5% (25/82) ; the sensitivity were 64.3% (18/28) ,71.9% (23/32) and 9.5% (6/63)respectively. The specificity was 100% for all the three substances. This research developed a new method by using indirect agglutination reaction to detect A, B and H substance in saliva. It also provides method for the measurement of salivary mucin MG1 qualitatively by using the corresponding specific antibody.
出处 《标记免疫分析与临床》 CAS 2009年第3期163-166,共4页 Labeled Immunoassays and Clinical Medicine
关键词 高相对分子质量粘蛋白MG1 间接凝集反应 血型物质 唾液 含钙微粒 High-molecular-weight salivary mucin (MG1) Indirect agglutination reaction Blood group substance Saliva Calcium-containing particle
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  • 1George D L,Michael T B,Daniel R P,et al.Mucin Apoprotein Expression in COPD[J].Chest,2002,121:166-168.
  • 2Rose M C,Voynow J A.Respiratory tract mucin genes and mucinglycoproteins in health and disease[J].Physiol Rev,2006,86:245-249.
  • 3Tabak L A.Structure and function of human salivary mucins[J].Crit Rev Oral Biol Med,1990,1:229-233.
  • 4Tabak L A.In defense of the oral cavity structure,biosynthesis,and function of salivary mucins[J].Annu Rev Physiol,1995,57:547-551.
  • 5Prakobphol A,Leffler H,Fisher S J.The high-molecular-weight human mucin is the primary salivary carrier of ABH,Le(a),and Le(b) blood group antigens[J].Crit Rev Oral Biol Med,1993,4:325-327.
  • 6Lloyd K O,Yin B W.MUC-6 mucin is a major component of "blood group substance" from human ovarian cyst fluid[J].Biochim Biophys Acta,2000,1474(3):410-414.
  • 7Thomsson K A,Benjamin L,et al.MUC5B glycosylation in human saliva reflects blood group and secretor status[J].Glycobiology,2005,15(8):791-796.
  • 8Fisher S J,Prakobphol A,Kajisa L,et al.External radiolabelling of components of pellicle on human enamel and cementum[J].Arch Oral Biol,1987,32(7):509-513.

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