急性化脓性腹膜炎相关性腹水对大鼠肝脏的损伤作用研究

Study on The Effect of Acute Suppurative Peritonitis Associated Ascitic Fluid on Experimental Liver Injury of Rats

目的探讨急性化脓性腹膜炎相关性腹水(ASPAAF)诱导大鼠肝脏损伤的作用及其机理。方法将32只SD大鼠(雌雄不拘)随机分为2组:ASPAAF组(n=16)和对照组(n=16),分别向腹腔内注射ASPAAF和生理盐水,每只8 ml。于腹腔注射后6 h和12 h分批处死大鼠,每时间点8只。检测血清内毒素及TNF-α水平和肝功能(AST、ALT和STB);检测肝脏组织TNF-α水平;光镜下观察肝脏组织损伤情况。结果于腹腔注射后6 h和12 h,ASPAAF组血清内毒素、TNF-α、AST、ALT和STB水平以及肝脏组织TNF-α水平均较对照组高(P<0.05);以上指标在ASPAAF组组内随时间的增加而显著增高(P<0.05);ASPAAF组肝脏组织细胞出现水肿,甚至点状坏死,6 h和12 h间无明显差别,对照组肝脏组织细胞正常。结论ASPAAF能引起大鼠肝脏损伤,内毒素和TNF-α在这一损伤过程中发挥了重要作用。

Objective To study the effect and intrinsic mechanism of acute suppurative peritonitis associated aseitic fluid （ASPAAF） on experimental liver injury of rats. Methods Thirty-two male or female Sprague-Dawley （SD） rats were randomly divided into two groups： ASPAAF group （n=16） and control group （n= 16）, in which 8 ml ASPAAF or normal saline （NS） were injected into the peritoneal cavity, respectively. The rats were killed at each time intervals after peritoneal cavity injection （6 h and 12 h） respectively in two groups and specimens were made to detect the levels of serum TNF-α, endotoxin and liver function （AST, ALT and STB）. The level of TNF-α in liver tissues was measured. The pathological change of liver was observed by microscope. Results The levels of TNF-α, endotoxin, ALT, AST and STB in serum and the levels of TNF-α in liver tissues at different time points were markedly higher in ASPAAF group compared with those in control group （P〈0. 05）, and these indexes increased with increasing time in ASPAAF group （P〈0.05）. In ASPAAF group, hepatic tissue appeared hydrops, even spotty necrosis and the changes at 6 h and 12 h were not obvious different. No abnormal pathological change of hepatic tissue was found in control group. Conclusion ASPAAF can induce the injury of the liver in rats, which may involved in TNF-α and endotoxin.