Survivin、PTEN在胃癌中的表达及临床意义

Expression of Survivin and PTEN in gastric cancer and its clinic significance

目的探讨Survivin、PTEN与胃癌的发生、发展、浸润转移及预后的关系。方法运用原位杂交、免疫组化及TUNEL法检测Survivin、PTEN在胃癌中的表达以及细胞凋亡情况。结果survivin在胃癌中的表达率明显高于癌旁正常胃组织（P〈0．01）；并且在临床分期较高、组织分化较差的胃癌中Survivin表达率较高（P〈0．01，P〈0．05）。而PTEN在胃癌中的表达率明显低于癌旁正常胃组织（P〈0．01）；在临床分期较高、组织分化较差的胃癌中PTEN表达率较低（P〈0．01）。此外，胃癌中survivin与凋亡指数呈负相关（r=-0．861，P〈0．01），PTEN与凋亡指数呈正相关（r=0．832，P〈0．01）。结论Survivin可以作为早期胃癌检测及胃癌愈后判别的标志物之一；PTEN基因的失活可能促进了胃癌的发生、发展和浸润转移的进程。

Objective To investigate the relationship among Survivin, PTEN and the occurrence, development, soakage and prognosis of gastric cancer. Methods The in-situ hybrization technique, immunochemistry and TUNEL method were used to detect the expression of survivin mRNA, PTEN protein and apoptotie cell. Results The positive rate of survivin expression in gastric cancer was obvious higher than that in para - cancer tissue（ P 〈 0. 01 ）. Moreover, survivin expression rate in advanced clinical stage and low differentiation gastric cancer degree was higher, compared with para - cancer tissue （ P 〈 0.01, P 〈 0. 05 ）. The positive rate of PTEN expression in gastric cancer was obvious lower than that in para -cancer tissue （ P 〈 0. 01 ）. The positive rate of PTEN expression in advanced clinical stage and low differentiation degree of tissue in gastric cancer was lower （ P 〈 0. 01 ）. Furthermore, survivin and apoptosis indexes manifested negative relationship in gastric cancer（ r = -0. 861, P 〈 0. 01 ）, while PTEN and apoptosis indexes manifested positive relationship（ r = 0. 832, P 〈 0. 01 ）. Conclusions Survivin could be used as a novel marker to detect gastric cancer. PTEN gene lost function, which maybe promote the origin, growth, soakage and metastasis of gastric cancer.