高压氧对荷瘤大鼠的影响

The effects of hyperbaric oxygen on the life-span of tumor-bearing mice

目的探讨高压氧对荷瘤动物寿命的影响。方法将28只雄性Balb／c小鼠随机分为腹腔肿瘤组（肿瘤细胞接种于腹腔，暴露于常压空气）、腹腔肿瘤高压氧组（肿瘤细胞接种于腹腔，行高压氧暴露）、背部肿瘤组（肿瘤细胞接种于背部，暴露于常压空气）和背部肿瘤高压氧组（肿瘤细胞接种于背部，行高压氧暴露），每组7只。各组小鼠于相应部位接种S—180癌细胞后，分别暴露于常压空气或高压氧中，对其寿命和皮肤破溃时间进行观察和记录。结果腹腔肿瘤组动物存活时间为（27．6±4．5）d，腹腔肿瘤高压氧组动物存活时间为（24．0±2．9）d，2组差异无统计学意义（P〉0．05）；背部肿瘤组动物存活时间为（63．0±21．8）d，背部肿瘤高压氧组动物存活时间为（35．1±8．9）d，2组差异有统计学意义（P〈0．01）；背部接种肿瘤的2组动物分别有4只出现背部皮肤破溃，背部肿瘤组破溃的时间为（18．5±3．3）d；背部肿瘤高压氧组破溃的时间为（24．7±5．1）d，2组差异有统计学意义（P〈0．01）。结论高压氧暴露可明显缩短背部接种肿瘤小鼠的存活时间，但对腹腔接种肿瘤小鼠的寿命无明显影响（P〉0．05）。高压氧暴露可推迟肿瘤生长引起的皮肤破溃。

Objective To explore the influence of hyperbaric oxygen （HBO） on the life-spans of tumor- bearing mice. Methods Twenty-eight male Balb/c mice were randomly and evenly divided into： a celiac-tumor group （inoculated with tumor cells in the abdominal cavity）; a celiac-tumor-HBO group （inoculated and then exposed to hyperbaric oxygen） ; a back-tumor group （inoculated under the skin of the back） ; and a back-tumor-HBO group （inoculatedunder the skin of the back and then exposed to hyperbaric oxygen）. S-180 carcinoma cells were inoculated, and then HBO was administered once a day. The life-spans and any skin ulceration were observed. Resuits The average life-spans were （27.6 ± 4.5 ） days in the celiac-tumor group, （ 24.0 ±2.9） days in the celiactumor-HBO group, （63.0± 21.8） days in the back,tumor group and （35.0 ± 8.9） days in the back-tnmor-HBO group. The difference in average life-span between the celiac-tumor group and the celiac-tumor-HBO group was not significant. The difference in life-span between the back-tumor group and the back-tumor-HBO group was significant. Ulcers occurred in 4 mice in the back-tumor group and 4 in the back-tumor-HBO group. The times of ulcer formation were significantly different between the back-tumor group and the back-tumor-HBO group. Conclusions The life-spans of mice in the back-tumor-HBO group were shortened by HBO exposure, but life-spans in celiac-tumor-HBO group were not. Ulcer formation was postponed by HBO in the back-tumor-HBO group.