人参总皂苷合黄连小檗碱对慢性心衰大鼠心肌肌球蛋白重链的影响

Effects of total ginsenoside combined with berberine on cardiac myosin heavy chain in experimental rats with chronic congestive heart failure

目的:探讨人参总皂苷(GS)合黄连小檗碱(Ber)对慢性心衰大鼠心肌肌球蛋白重链(α-MHC,β-MHC)表达的影响.方法:连续12 d皮下注射较大量异丙肾上腺素(ISO)建立慢性心衰大鼠模型,ISO总量80 mg/kg,随机分为模型组、GS+Ber组、卡托普利组、参麦注射液组,另设对照组.GS+Ber组各以20 mg/(kg.d)灌胃,卡托普利组以45 mg/(kg.d)灌胃,参麦注射液组以5 mL/(kg.d)腹腔注射,模型组和对照组以蒸馏水灌胃.4 wk后检测各组血流动力学指标、心脏质量指数,实时荧光定量PCR检测α-MHC,β-MHC mRNA表达.结果:对照组与各组相比,左室内压峰值(LVSP)、左室等容收缩期压力上升最大速率(+dp/dtmax)、左室舒张期压力下降最大速率(-dp/dtmax)以及α-MHC mRNA表达明显下降,左室舒张末压(LVEDP)、心脏质量指数以及β-MHC mRNA表达显著升高(均P<0.01);GS+Ber组、卡托普利组与模型组相比,LVSP,±dp/dtmax,α-MHC mRNA表达明显升高,LVEDP、心脏质量指数以及β-MHC mRNA表达显著降低(P<0.01或P<0.05).结论:GS+Ber能增加慢性心衰大鼠心肌细胞内α-MHC mRNA表达,降低β-MHC mRNA表达,为GS+Ber改善心功能和心室重构的主要机制之一.

AIM： To study the effects of total ginsenoside（GS） combined with berberine （Ber）on the expression of cardiac myosin heavy chain in cardiac cells of chronic congestive heart failure rats. METHODS： Animal models of heart failure were induced by subcutaneous injection of a large dose of ISO （80 mg/ kg） for 12 d and were randomly divided into model group, GS ＋ Ber group, captopril group and shenmai injection group. Some other rats served as control group. GS ＋ Ber group were treated with total ginsenoside and berberine （each 20 mg/kg; once per day） by gavage, captopril group were given captopril （45 mg/kg; once per day）by gavage, shenmai injection group were injected intraperitoneally with shenmai injection （5 mL/kg ; once per day） and model group and control group were given distilled water by gavage. After 4 weeks, hemodynamic indexes and cardiac weight index were examined and α-MHC and β-MHC mRNA expressions were detected by real-time quantitative PCR. RESULTS： LVSP, ＋ dp/dtmax, -dp/dtmax and α-MHC mRNA expressions were lower and LVEDP, cardiac weight index and β-MHC mRNA expressions were significantly higher in control group than those in other groups（P〈0.01）. Compared with those in model group, LVSP , ＋ dp/dtmax, -dp/dtmax and α-MHC mRNA expressions were higher and LVEDP, cardiac weight index and β-MHC mRNA expressions were significantly lower in GS ＋ Ber group and captopril group （P〈0.01 or P 〈0.05）. CONCLUSION ： Total ginsenoside combined with berberine can increase α-MHC mRNA expression but decrease β-MHC mRNA expression in cardiac cells of chronic congestive heart failure rats, which mac be one of the major mechanisms of improving heart function and ventricular remodeling.