地塞米松对大鼠坐骨神经损伤后脊髓神经元碱性成纤维细胞生长因子的影响

Dexamethasone improves expression of basic fibroblast growth factor in neurons of spinal cord following sciatic nerve injury in rats

目的:周围神经损伤的再生修复是神经科学研究的重要课题。碱性成纤维细胞生长因子(bFGF)在神经系统的生长发育过程中起重要作用。地塞米松可用于治疗神经损伤。文中通过制备大鼠坐骨神经钳夹损伤模型,观察地塞米松对大鼠坐骨神经损伤后脊髓运动神经元bFGF的影响。方法:56只Wistar大鼠分为4组。神经损伤组:大鼠右侧股外侧切口,钳夹右侧坐骨神经造成神经损伤;地塞米松组:神经损伤后即刻局部肌肉间隙内注射地塞米松0.5 mg/(kg.d);等渗盐水组:在神经损伤后等时间点注射等量等渗盐水;正常对照组:不做任何处理。用免疫组化技术观察脊髓前角运动神经元bFGF的变化。结果:地塞米松明显提高神经损伤后脊髓运动神经元bFGF的表达。结论:地塞米松通过增强坐骨神经钳夹损伤后脊髓前角运动神经元内bFGF的表达,促进受损神经元的功能恢复。

Objective ： The regeneration and repair of peripheral nerves are focuses of neurological studies. The （bFGF） may play an important role in the development of the neural system. Dexamethasone is usually employed in the management of neural injuries. The aim of this study is to establish a rat model, and investigate the effect of Dexamethasone on the expression of bFGF in the neurons of the spinal cord following sciatic nerve injury. Methods： Fifty-six male Wistar rats were randomly divided into 4 groups ： a nerve injury group, with sciatic nerve injury induced by clamping the nerve in the right ; a dexamethasone group, immediately injected with dexamethasone [0.5 mg/（kg/d） ] in the injury side; a saline group, given saline only ; and a control group, rats without sciatic nerve injury. Changes of bFGF in the motor neurons of the L4 - L6 spinal cords were observed by immunohistochemistry. Results ： Dexamethasone obviously upregulated the expression of bFGF in the motor neurons of the spinal cord. Conclusion ： Dexamethasone can improve the expression of bFGF in the motor neurons of the spinal cord after sciatic nerve injury, and contribute to the recovery of the function of the injured neurons.