5种结核分枝杆菌特异蛋白质的抗原性分析

Evaluation of antigenicity of 5 Mycobacterium tuberculosis specific proteins

目的评价14、16、38kD、mtb81和ESAT-6等5种结核分枝杆菌特异蛋白质的抗原性。方法利用14、16、38kD、mtb81和ESAT-6等5种重组蛋白建立ELISA方法,检测120份结核病人、30份非结核呼吸道感染病人和30份健康人血清中相应抗体。结果ELISA显示5种结核杆菌特异性蛋白质的特异性均高于95%;敏感性和准确性则不尽相同,其中38kD的检测敏感性最高,为80.8%;14、16kD、mtb81和ESAT-6的检测敏感性分别为52.5%、68.3%、35.8%和44.2%。结论5种结核杆菌特异性蛋白质均具有不同程度的抗原性。进一步提高抗原或抗体的检测灵敏度将有利于结核病的诊断。

Objective To evaluate antigenicity of 5 Mycobacterium tuberculosis specific proteins including 14, 16, 38 kD, Mtb81 and ESAT-6. Methods All the recombinant proteins of 14, 16, 38 kD, mtb81 and ESAT-6 were generated, and employed in ELISA to detect the antibodies in serum samples of 120 TB patients, 30 non-TB patients and 30 normal human. Results The results showed that all 5 proteins gave above 95% specificity, while 38 kD showed 80.8% sensitivity which was more sensitive than any other assays using 14, 16 kD, Mtb81 and ESAT-6 recombinant pro- teins. Conclusion All 5 specific proteins of Mycobacterium tuberculosis showed different levels of antigenicity. It was useful for TB diagnosis, and improved assay might be helpful to detect the antigens or antibodies against them.