靶向树突状细胞表面分子DEC-205的长循环免疫脂质体稳定性影响因素的数学模型

Factors Affecting the Stability and its Mathematical Modeling of Long-Circulating Immunoliposome Targeting to Dendritic Cell Surface Molecule DEC-205

在多相分散体系的动力稳定性理论基础上,设计了以DEC-205单抗为导向、以脂质体为载体、DCs为靶点的免疫策略,将包裹药物的脂质体特异性地靶向树突状细胞(DCs).对影响DEC-205的免疫脂质体稳定性的各种因素如粒径分布等进行考察分析,对条件进行优化,构建了经DEC-205单抗靶向DCs的免疫脂质体模型.模型的成功构建为进一步研究抗原靶向DEC-205受体后的体内免疫应答情况提供了工作基础,有望开发一种新型DCs疫苗应用于临床.

We designed a immunization strategy which enable the liposome to deliver encapsulated drugs or antigen to dendritic cells efficiently, based on the dynamic stability theory of multiphase dispersed system： used DEC-205 monoclonal antibody as guidance; liposome as carrier and DCs as target. We investigated and analyzed the effect of particle size distribution on stability of the DEC-205 Immunoliposome, and optimized the condition. The anti-DEC-205- immunoliposomes （anti-DEC-205 iLPSM）, as drug carriers, can specifically recognize dendritic cells and deliver FITC-dextran into the cytoplasm. The construction of an anti-DEC-205 iLPSM model lays solid foundations for further work researching immune response after antigen targeting to DEC-205 receptors in vivo. Anti-DEC-205 iLPSM could become a new type of DC vaccine.