摘要
目的探讨多西他赛单用及与野生型p53基因联合应用对宫颈癌Hela细胞的抑制作用。方法Hela细胞分两组培养,p53转染实验组(p53-Hela组)和空白对照组(Hela组),野生型p53基因表达用逆转录-多聚酶链反应鉴定。多西他赛作用于p53-Hela细胞及Hela细胞,24小时与48小时后形态学观察并使用活细胞计数试剂盒检测吸光度值OD450,计算细胞抑制率。结果用逆转录-多聚酶链反应法检测到p53-Hela细胞的p53mRNA表达,与对照组Hela细胞的p53mRNA表达相比,差异有统计学意义(t=-17.504,P〈0.01)。多西他赛对p53-Hela及Hela细胞作用时,不同时间都有抑制作用(FP53-Hela=53.500,P〈0.01;FHela=430.225,P〈0.01),该抑制作用呈剂量依赖性和时间依赖性。结论多西他赛单独应用时可对宫颈癌Hela细胞产生抑制作用,与野生型p53基因联用时,其药物抑制作用增强。
Objective To investigate inhibitory effects of docetaxel alone and docetaxel combined with wild type p53 (wt t)53 ) on proliferation of human cervical cancer cells-Hela cells. Methods The Hela cells were divided into two groups : wt p53 transfected Hela cells group(p53-Hela group, obtained by using lipofection-mediated gene transfection method) and blank control group (not transfected with wt p53). The expression of wt p53 was deteced by using reverse transcription polymerase chain reaction(RT-PCR) method. Docetaxel acted on Hela cells and p53-Hela cells. After 24 hours and 48 hours, the morphology of the cells in the two groups were observed respectively and OD450 were tested by using cell counting kit-8 (CCK-8) to caculate the inhibition rate. Results After transfection with wt p53, the expression of p53mRNA in p53-Hela group was increased, and there was a significant difference as compared with that in Hela group ( t = - 17. 504, P 〈 0.01 ). Docetaxel exerted an inhibitary effect on the Hela cell line in the two groups at all action times (FP53-Hela = 53. 500,P 〈 0.01 ;FHela = 430. 225, P 〈 0.01 ). This cellular inhibitory effects showed dose and time dependent manners. Conclusion Docetaxel alone exerts an inhibitory effect on Hela cells and this inhibitary effect could be increased when docetaxel is combined with wt p53.
出处
《中国妇幼健康研究》
2009年第4期417-419,共3页
Chinese Journal of Woman and Child Health Research