摘要
目的调查血液培养中病原菌的菌群分布及耐药趋势。方法1995/2004血液培养标本用荧光全自动血液培养仪(Bactec9120)进行培养,阳性标本用全自动微生物鉴定仪(Vitek32)进行鉴定,药敏采用KB法。结果在3104份血液培养标本中分离出病原菌331株,阳性检出率为10.7%。病原菌以革兰阳性(G+)需氧球菌居首位(50.3%),革兰阴性(G-)需氧杆菌次之(44.7%),真菌占3.0%,厌氧菌占1.1%。血液培养中的G+球菌对万古霉素和亚胺培南较为敏感,G-杆菌对亚胺培南、舒普深、丁胺卡那较为敏感。结论血液培养病原菌以G+球菌为主,G-杆菌次之;儿童血液培养病原菌以葡萄球菌属为主;亚胺培南对G+球菌和G-杆菌均具有较高的敏感率。
Objective To investigate the distribution and antimicrobial resistance of pathogens isolated from blood specimens in patients with fever in our hospital from 1999-2007, Methods Blood specimens were cultured by Bactec 91207 Fluorescent Instruments. The colonies of the specimens yielding growth were identified by the Vitek32 Instruments and KB method was used for the drug sensitivity tests. Results Three hundred and thirtyone bacteria strains were isolated from a total of 3104 blood culture specimens, with a positive rate of 10.7%. Of the identified bacteria, G+ cocci accounted for 50. 3%, G-bacilli accounted for 44. 7%, fungi accounted for 3.0%, and anaerobian accounted for 1.1%. The susceptibility rates of G-bacilli to Imipenem, Amikacin and cefoperazone/sulbactam were high. The susceptibility rate of G+ cocci to vancomycin and Imipenem were also high. Conclusion G+ cocci prevail over G-bacilli in the blood specimens. The staphylococci are the chief bacteria in the childrens blood specimens. Both G~ cocci and G-bacilli are sensitive to Imipenem.
出处
《中国实用医药》
2009年第8期45-46,共2页
China Practical Medicine
关键词
血液培养
病原菌
抗药性
多药
分离鉴定
TANG Jian-zhou. (Yong-zhou People' s Hospital, Hunan 425000, China)