日粮中缺硒对鸡空肠肥大细胞组织化学和超微结构的影响

Histochemical and Ultrastructural Effects on Mast Cells of Jejunums in the Chicken of Selenium Deficiency on the Dail Food

为探讨硒缺乏对鸡空肠中肥大细胞(MC)的形态学和超微结构的影响,将10只鸡随机分成低硒试验组(饲喂低硒日粮0.032mg/kg)和正常对照组,在75日龄时断头处死立即取出空肠,进行甲苯胺蓝、长时间甲苯胺蓝、阿利新蓝-藏花红染色、阿利新蓝-高碘雪夫氏反应和醛品红-阿利新蓝5种染色并进行透射电镜观察。结果显示,采用不同染色方法所获得的MC数量、大小、形态、颜色及颗粒的黏多糖成分均不相同。电镜下,MC细胞胞浆内充盈大量的特征性颗粒,颗粒内容物呈均质状,电子密度高低不等,核圆形或椭圆形,胞质中可见高尔基复合体和较多的线粒体;试验组可见MC数量显著增加并与变性细胞紧密相邻,异染色质增多,核偏位或固缩,胞质稀疏,同时可见MC内颗粒内容物释放后的空隙或空泡。因此,组化染色提示肥大细胞存在不同亚型;试验组MC数量明显增加,这提示MC是重要的免疫细胞之一,在机体的免疫调节中发挥着重要的作用。

To study histochemical and ultrastructural effects of Se deficiency on mast cells of jejunums in the chicken. 10 chicks were randomly divided into two groups （control group and Se deficiency group）. The chicks were killed by decapitation at 75 d and then the jejunums were collected. Microstructure and ultrastructure of jejunums were observed using routine toluid- ine blue（RTB）,long toluidine blue（LTB）,alcian blue safranin （AB-S）, aldehyde fuchsin alcian blue（AF-AB）, alcian blue-periodic acid Sehiff reaction（AB-PAS）and transmission electron microscope technology. Results showed that these mast cells had heterogeneity in quantity,shapes,sizes,colouration,mucopolysaccbarides with the different staining methods, it may hint that these cells were divided into different subtypes. The amount of mast cells inereased obviously in Se deficiency group, it shows that mast cells are one of the substaintial immunoeyte and play an important part in immunological regulation. Under transmission electron microscope,abundant homogeneous cytoplasmic granules of different electron density were seen in cytolymph, nucleolus was spherical or oval, eomplexs of Golgi and major mitochondrias were observed in periplast. In Be difeeiency group,it was seen that MC increased significantly and were close together with degenerating cells,heterochromatin increased, nucleus became unsymmetric, periplast was sparse and vacuoles were observed after cytoplasmic granules released the content.