摩杂一代水牛脑垂体前叶的超微结构研究

Ultrastructure Study of the Prehypophyseal Cells in F1 Hybrid-marrah Buffalo

利用超薄切片对摩杂一代水牛脑垂体前叶的腺细胞进行透射电镜观察,发现5种腺细胞各有其超微结构特征:促性腺激素细胞胞体呈圆形,直径为7.6～9.0μm;分泌颗粒分为明显的大小2种颗粒,且电子密度高。促甲状腺激素细胞的直径为6.0～7.5μm;分泌颗粒少而最小,且大小无显著差异,多沿质膜分布;细胞核小,多位于中央。促肾上腺皮质激素细胞多在邻近垂体神经部的腺体前叶中,细胞核不规则,常偏向一侧,分泌颗粒常于细胞周边或一端;胞体直径为7.1～8.5μm。促生长激素细胞数量多,核大而圆,分泌颗粒多且均一,充满胞质。催乳素细胞胞体最大,直径为11.2～13.4μm,数量多,核小,常偏于一侧;分泌颗粒多而大小不等,充满胞质。旨在为研究摩杂一代水牛生殖泌乳的内分泌调控机制提供形态学基础资料。

Under transmission electron microscope, the prehypophyseal cells in F1 hybrid-marrah buffalo have five marked cells types in common, their ultrastructural features appeared characteristically. Gonadotrophs are round in shape, which about 7.6 to 9.0 μm in diameter; the size of secretory granules is either big or small clearly, which has high electron density. Thyrotroph is about 6.0 to 7.5μm in diameter and contains irregular nuclei in the centre mostly; their secretory granules are generally minor and tiny,distributed in the vicinity of plasmalemma. Adrenocorticotrophic hormone（ACTH）cells are ovoid in shape and 7.1 to 8.5μm in diameter; irregular nuclei always is side incline; their secretory granules occurred in cytoplasmic periphery or one side. Somatotrophs are round, containing rounded nuclei and numerous secretory granules. Lactotrophs are almost the biggest cells in pituitary, numerous cells of various size are full of periplast. The results aimed to provide the morphology basic data for controlling reproduction endocrine in F1 hybrid-marrah buffalo.