猪瘟病毒RT-PCR核酸检测与ELISA抗原检测试验应用研究

The Applied Research on the RT-PCR Nucleic Acid Detection and ELISA Antigen Detection Kit of CSFV

本试验根据NCBI公布的猪瘟病毒石门毒株E2基因序列,设计、合成了1对检测猪瘟病毒E2基因的PCR引物。以120份疑似猪瘟病料为试验材料,使用RT-PCR核酸检测与ELISA抗原检测2种方法,分别用这2种方法检测疑似病料,并且比较这2种方法在猪瘟病料检测中的实际差别。通过猪瘟病毒RT-PCR核酸检测结果及ELISA抗原检测结果相互印证,为云南猪瘟的诊断及防制提供了技术支持。

In order to analyze the differences between RT-PCR and ELISA antigen detection methods in classical swine fever virus detection, 120 clinical samples were detected by the two methods. According to the study released by the NCBI swine fever virus strain Shi-men E2 gene sequence, design and synthesis of a pair of detection of classical swine fever virus E2 gene PCR primer. Submitted to the 120 suspected swine fever disease compound extracted total RNA, after one-step RT-PCR amplification of E2 gene, checked out 32 samples were expected to contain swine fever antigen, positive rate was 26.7 %. ELISA antigen detection kit checked out 38 samples were expected to contain swine fever antigen, positive rate was 31.7%. One-step RT-PCR detected 3Z positive samples were also positive in the detection of ELISA. The establishment of classical swine fever virus RT-PCR detection of nucleic acid and antigen detection ELISA. Pathogen detection of classical swine fever supplied technical support for the provision of Yunnan.