摘要
研究不同灭菌处理、不同基本培养基、不同激素种类及浓度对粗勒草"黑美人"和"银皇后"两品种茎段不定芽诱导的影响,结果表明:"黑美人":以75%酒精1min+0.1%HgCl2 20min的灭菌效果最佳,污染率为25.56%;以MS+6-BA 4.0mg/L+NAA 0.05mg/L的诱导效果最好;"银皇后":以75%酒精1min+0.1%HgCl2 22min的灭菌效果最佳,污染率为29.6%;以MS+6-BA 4.0mg/L+NAA0.1mg/L的诱导效果最好。
The effect of different sterilization treatments, basic mediums, hormone kinds and concentrations on bud inducement of two Aglaortema commutatum varieties was studie.The result indicates that in A gloonema commutatum "Treubii" : 75%C2H5OH 1 min+0.1% HgCl2 20mins had the best sterilization effect, whose contamination rate was 25.56%, and MS+6-BA 4.0mg/L +NAA 0.05mg/L had the highest inductivity. While in A glaonema commutatum "Malay Beauty" : 75% C2H5OH 1 min +0.1% HgCl2 22mins had the best sterilization effect, whose contamination rate was 29.6% ,and MS +6- BA4.0mg/L+NAA0.1mg/L had the highest inductivity.
出处
《广西农学报》
2009年第3期19-22,共4页
Journal of Guangxi Agriculture
关键词
粗勒草
茎段培养
不定芽诱导
污染率
诱导率
Aglaonema spp
stem culture
bud inducement
contamination rate
inducement rate