一株脱色真菌的鉴定及脱色特性的初步探讨

Preliminary Discuss on Identification and Characterization of One Decolorizing Fungus

从废水环境中分离筛选到一株高效染料脱色真菌,根据形态学及显微特征初步鉴定为泡盛曲霉(Aspergillus awamori),命名为Asaw117;从偶氮类、蒽醌类和氧醌类中选取8种不同染料进行脱色分析表明,该菌株对0.1g/L蒽醌类染料还原蓝RSN的脱色率可达100%;采用不同培养基及不同种类碳氮源进行试验比较,菌株在查氏培养基中生长慢,但脱色效果最好,在马铃薯培养基中生长旺盛,脱色效果次之。此外,菌株Asaw117能利用还原蓝RSN作为氮源,但不能利用其为碳源;几种碳氮源组合实验中,菌株在蔗糖、硝酸铵组合的组氏培养基脱色效果为好。因此对处理印染废水具有较好的应用潜力。

According to morphological and microscopic characteristics, a high-efficient decolorizing fungus, the strain Asaw117, was identified as Aspergillus awamori. Selecting eight different dyes from Azo dyes, anthraquinones dyes and oxygen Quinones dyes, the decolorizing assays of various dyes showed that the strain Asaw 117 was the highest decolorizing potential to 0.1 g/L Vat Blue RSN, the discoloration rate up to 100 percent. Comparing to different kinds of medium and several of carbon and nitrogen sources, the strain had the best decolorizing efficient although grew slower in the Czapek medium, otherwise, grew quicker and decolorizing efficient lower in the PDF medium. It could use Vat Blue RSN as a nitrogen source, but not as a carbon source. The medium composing of saccharose and ammonium nitrate as carbon and nitrogen sources was decolorizing potential markedly during different combinations of carbon and nitrogen sources. So the strain has good potential for the dyeing wastewater treatment.