粳稻野败型细胞质雄性不育恢复系SWR78的恢复基因定位

Mapping of Fertility-Restoring Gene for Wild-Abortive Type Cytoplasmic Male Sterility in a japonica Rice Restorer Line SWR78

利用野败(WA)型粳稻广亲和不育系苏秋A和广亲和广谱型恢复系SWR78配组,根据F2与BC1F1群体的育性分离情况,初步推测WA型苏秋A的育性恢复至少由3对基因控制。选取F2群体中无染色花粉植株,采用隐性基因组分析法进行恢复基因定位,将其中1个主效基因Rf4定位于第10染色体长臂上,与标记RM5629、RM5373、STS10-17和STS10-18分别相距0.17、0.03、0.03和0.07 cM。Rf4位于标记RM5373与STS10-17之间,两标记间的物理距离为78 kb。

According to the fertility segregation of F2 and BC1 F1 populations derived from a cross between Suqiu A with wild-abortive type cytoplasmic male sterility （CMS） and SWR78（a japonieal rice restorer line）, it could be presumed that the fertility restoration of this cross was controlled by three genes. Then, individuals with absolutely abortive pollen of F2 population were used to map the fertility-restoring gene. The R f4 gene was mapped on the long arm of chromosome 10 flanked by the markers RM5629, RM5373, STS10-17 and STS10-18 at the distances of 0.17, 0.03, 0.03 and 0.07 cM, respectively. The R f4 gene was localized between RM5373 and STS10-17, and the physical distance of the interval between the two mark- ers was about 78 kb.