慢性牙周炎患者白细胞介素-8的检测

Measurements of IL-8 in patients with periodontitis

目的探讨牙龈组织中IL-8与慢性牙周炎的关系。方法采用双抗夹心酶联免疫吸附法(ELISA)测定20例慢性牙周炎患者与20例正常对照者龈沟液(GCF)及血清中的IL-8含量,并用免疫组化和原位杂交法,分析IL-8的检出率及IL-8分泌细胞在牙龈组织中的分布。结果牙周炎患者GCF中IL-8含量明显高于对照组GCF中的含量(P<0.01)及牙周炎患者血清中的含量(P<0.01);慢性牙周炎患者中的IL-8的检出率均高于正常对照者,IL-8阳性细胞分布于牙龈的上皮和结缔组织中。结论IL-8主要来源于牙龈组织局部,IL-8参与了牙周炎的病理过程。

Objective To study the relation of interleukin-8 （IL-8） in periodontitis. Methods IL-8 contents in gingival crevicu- lar fluid （GCF） were measured in periodontitis and healthy controls with sandwich enzyme-linked immunosorbent assay （ELISA）. In addition, IL-8 contents in serum were studied in periodontitis and healthy controls at the same time. And by immunohistochemical and in situ hybridization analysis of IL-8 detection rate and IL-8 secretion of cells in the gingival tissue distribution. Results IL-8 level in GCF was markedly higher in periodontitis than in healthy controls （P^0. 001） and than in serum of periodontitis （P〈7 0. 001）. Patients with chronic periodontitis in IL-8 detection rate were higher than normal controls,IL-8-positive cells distributed in the gingival epithelium and connective tissue. Conclusion IL 8 mainly come from the gingival tissue,IL-8 involved in the pathologi cal process of periodontitis.