摘要
基于纳米金标记和金增强表面吸附伏安分析的新型电化学免疫传感器。以人免疫球蛋白G(hIgG)为模型分析物,使固定化抗体与分析目标物hIgG及纳米金标记的hIgG抗体结合于电极表面,通过金沉积使电极表面的纳米金颗粒直径增大。由于金增强时间在几分钟内就可完成,和传统的银增强相比,缩短了增强时间,加快了分析速度。研究结果表明,该法灵敏度高,选择性好,重现性好,操作简便,可望成为一种有潜力的新型高灵敏度免疫传感技术。
Based on nano-gold mark and the enhanced surface analysis of a new type of electrochemical voltage immunosensor. Human immunoglobulin G (h IgG) model for the analysis of the material so that the immobilized antibodies and analysis of the target h IgG and the gold nano-h IgG antibody binding to the electrode surface, so that the gold electrodes deposited on the surface of gold nano-particles in diameter by Great. As the time to enhance the gold will be completed in a matter of minutes, and strengthen the traditional silver, shortened the time to strengthen and speed up the analysis of speed. The results show that the law of the high sensitivity and selectivity, reproducibility, and easy to operate, is expected to have the potential to become a new type of highly sensitive immune sensing technology.
出处
《化学传感器》
CAS
2009年第2期18-24,共7页
Chemical Sensors
关键词
纳米金
生物分子固定
电化学免疫传感器
gold nano-particals
biomolecule immobilization
immunosensor of electrochemicai