腹腔注射板蓝根多糖对中华鳖肝脏抗氧化酶活性和丙二醛含量的影响

Effect of intraperitoneal injection of indigowoad root polysaccharide on antioxidant enzyme activity and MDA content in the liver of Pelodiscus sinensis

研究了腹腔注射板蓝根多糖对中华鳖肝脏抗氧化性的影响。实验共分4组:每天注射1.5mg/只组、3mg/只组、6mg/只组和注射生理盐水对照组,连续注射3d。结果表明:3个板蓝根多糖处理组均能提高SOD、CAT、GSH-PX的活性,降低MDA的含量,但存在着差异。与对照组相比,3个处理组均能显著提高CAT的活性,而且随注射剂量的增多,活性增强。3mg/只、6mg/只剂量组能显著增加GSH-PX活性,显著降低MDA含量,但3mg/只剂量组影响最大。而1.5、3mg/只剂量组对SOD的影响不显著,6mg/只剂量组影响最大而且差异极显著。提示板蓝根多糖能增强中华鳖肝脏的抗氧化性。

The effect of intraperitoneal injection of indigowoad root polysaccharide （IRPS） on the oxidation resistance was studied in the liver of Pelodiscus sinensis. There were four groups in this experiment and every group was continuously injected for three days. The first three groups were injected with 1.5 mg IRPS,3 mg IRPS, and 6 mg IRPS respectively. The fourth group was injected with physiological saline. The results showed that the activities of SOD, CAT and GSH-PX increased and MDA content decreased in the first three groups injecting with IRPS. However, there were some differences among them. Compared with the control, CAT activity significantly increased in the first three groups injected with IRPS and the activity increased with an increase of injection dosage. GSH-PX activity increased significantly and MDA content obviously decreased by injecting with 3 mg and 6 mg IRPS in P. sinensis, but the greatest effect was shown by injecting with 3 rag IRPS in P. sinensis. The effect on SOD activity was not significant by injecting with 1.5 mg and 3 mg IRPS in P. sinensis. The effect of the group by injecting with 6 mg IRPS in P. sinensis is the most significant difference. In a word, IRPS could improve the oxidation resistance in the liver of P. sinensis.