摘要
利用一步电沉积法,以含有环氧基团的γ-环氧丙氧丙基三甲氧基硅烷(GPTMS)为无机杂化试剂和功能性交联试剂,通过壳聚糖(Chitosan,CS)、辣根过氧化物酶(HRP)和葡萄糖氧化酶(GOD)分子中-NH2与环氧基团的反应,在金电极表面原位制备交联型有机-无机生物杂化膜,得到共固定HRP和GOD的新型双酶生物传感器.实验证实了这种有机-无机生物杂化膜在不同酸、碱条件下都具有高的稳定性和耐用性,克服了CS酸溶的不足,从而扩大了其使用范围.在葡萄糖检测中,交联型双酶传感器HRP-GOD/GPTMS/CS/Au比无交联的双酶传感器HRP-GOD/CS/Au具有更高的灵敏度、更宽的线性范围,其线性范围为1μmol/L~351μmol/L,检测限为0.3μmol/L.
Horseradish peroxidase (HRP) and glucose oxidase (GOD) bienzyme biosensor was constructed by in-situ formation of organic-inorganic biocomposite film based on one-step electrodeposition and covalent-coupled sol-gel process. The electrodeposition was performed in the solution containing functional inorganic precursor possessing epoxy group, γ-glycidoxypropyltrimethoxysiloxane (GPTMS), a biopolymer, chitosan (CS), and enzymes HRP and GOD. Covalent-coupled sol-gel process was formed by self-hydrolysis and self-condensation of GPTMS, and in-situ covalent cross-linking of CS, HRP, GOD through covalent reaction between amino groups and epoxy groups. The developed bienzyme biosensor presented high stability in acidic solution owing to the covalent-coupled organic-inorganic hybridization. Compared with non-hybrid HRP-GOD/CS/Au electrode, the bienzyme biosensor of HRP-GODIGPTMSICSIAu showed improved sensitivity and wider linear range for the determination of glucose. The linear response of the developed HRP-GOD/GPTMS/CS/Au biosensor for the determination of glucose ranged from 1 μmol/L to 351 μmol/L with a detection limit of 0.3 μmol/L.
出处
《中国科学(B辑)》
CSCD
北大核心
2009年第7期640-645,共6页
Science in China(Series B)
基金
国家自然科学基金项目(批准号:20775039
20775038)资助