摘要
单克隆抗体的特异性在导向药物治疗某些疾病的应用研究中起着重要的作用。尽管单克隆抗体对靶细胞具有特异性识别能力,但仍可能与一些无关细胞有着不同程度的交叉反应。本实验结果表明:(1)用浓度为10^(-8)M、10^(-9)M 的抗普通型急性淋巴细胞白血病单克隆抗体免疫毒素(79:ricin)处理骨髓细胞后,CFU-GM 的增殖分别受到抑制.(2)用79单克隆抗体阻断后,抑制作用明显减弱.(3)去除骨髓中79抗体阳细胞后,CFU-GM 的增殖也明显减弱。这表明79单克隆抗体不仅仅是一个识别普通型急性淋巴细胞白血病细胞的单克隆抗体,它还可能是一个识别部分骨髓粒单系造血祖细胞的单克隆抗体。
The specificity of monoclonal antibodies plays a very important role in the investigation of targetic drugs,Although monoclonal antibody can recognize specifically the target cells, many unrelated cells may also be recognized.Our experimental results demonstrate that anti- common acute lymphocyte leukemic cell antibody (79,CD_(10)) may react partly with granulo- monocyte progenitor cells of bone marrow.1.The proliferation of CFU-GM was inhibited after treating with 10^(-8)M and 10^(-9)M of 79:ricin.2.When bone marrow mononucleocytes were blocked by 79 antibody,the efficiency of 79:ricin decreased significantly for the pro- liferation of CFU-GM.3.Following removing 79 antibody positive cells from bone marrow, the proliferation of CFU-GM reduced,These results suggest that 79 antibody can not only recognize the common acute lymphocyte leukemic cell,but also the granulomonocyte progeni- tor cell of bone marrow.
出处
《军事医学科学院院刊》
CSCD
北大核心
1990年第3期208-211,共4页
Bulletin of the Academy of Military Medical Sciences
基金
国家863基金资助
