肠腺修复因子对受照射小鼠肠腺的修复再生作用

EFFECTS OF CRYPT REPAIR FACTOR ON THE REPAIR AND REGENERATION OF CRYPTS OF LIEBERKUHN IN IRRADIATED MICE

本文比较了用刮取法制备的正常小鼠肠腺细胞悬液及其加热制剂(100℃,15min)两者对受照射小鼠肠腺的修复再生作用。光镜、电镜观察及肠腺存活率测定的实验结果表明,无论是肠腺细胞悬液或其加热制剂,均具有同样的促进受照射小鼠肠腺修复再生的作用。因而证明,在刮取法制备的肠腺细胞悬液中,存在着某种或某些耐热的肠腺修复因子。而且证实,此种因子在小鼠腹腔内注射也是有效的。但关于它的化学性质尚需作进一步研究。

Heat-processed (100℃,15 min)and-unprocessed crypt cell suspensions,prepared by the scraping method from normal mice,were compared in their effects on the repair and regene- ration of crypts of Lieberkuhn in mice after irradiation with ^(60)Co γ-rays.Data obtained from light microscopical,electronmicroscopical observation and quantitation of crypt survival indica- ted that both the heat-processed and-unprocessed crypt cell suspensions were capable of faci- litating the crypt regeneration in the same manner and enhancing the crypt survival to the same extent in mice after irradiation.These results confirm that there exists,in the crypt cell suspension,a kind of heat-resisting crypt repair factor which is responsible for the new crypt formation,This crypt repair factor was proved to be effective via intraperitoneal injection, whereas its chemical entity remains to be identified.