活化转录调控因子2(ATF-2)在下颌骨髁突软骨细胞中调控bcl-2机制的研究

Role of activating transcription factor 2 in the growth of mandible condyle chondrocytes

目的:研究ATF-2通过bcl-2影响髁突软骨生长发育的机制。方法:应用体外培养原代小鼠髁突软骨细胞,原代细胞基因转染,蛋白印迹杂交等技术,阐明ATF-2调控bcl-2表达的机制。结果:外源性的ATF-2在髁突软骨细胞中,与bcl-2的调控子中CRE结合,增强bcl-2调控子的活性;当CRE位点的序列改变,ATF-2不能在髁突软骨细胞中对bcl-2调控子的活性产生影响。结论:ATF-2在软骨细胞中,通过影响有凋亡抑制作用的bcl-2的活性和表达,参与下颌骨髁突软骨的生长发育。

Objective： To study the role of activating transcription factor 2 （ATF-2） in the growth of mandibular condyle cartilage. Methods： Primary chondrocytes of condyle were cultured. Expression plasmid of ATF-2 and plasmid bcl-2 promoter were transfected into chondroeytes. Luciferase assay and Western blot were used. Results： The absence of ATF-2 in mandibular condyle chondrocytes resulted in a decline in bcl-2 promoter activity, reduction in bcl-2 protein level. Conclusion： The results strongly imply that ATF-2 is required for adequate bel-2 expression, and play a significant role in controlling growth plate ehondrocyte progression.