辛伐他汀对人牙周膜细胞增殖和分化的影响

Effects of Simvastatin on the proliferation and osteoblastic differentiation of human periodontal ligament cells

目的:研究辛伐他汀对人牙周膜细胞增殖和成骨分化的影响。方法:将第4代人牙周膜细胞在条件矿化培养液中诱导培养,同时加入不同浓度的辛伐他汀(10-9、10-8、10-7、10-6mol/L),噻唑蓝(methyl thiazolyl tetrazolium,MTT)法检测细胞增殖情况,4-硝基苯基磷酸二钠盐(4-nitrophenyl phosphate,hexahydrate,PNPP)偶氮法检测碱性磷酸酶(alkaline phosphatase,ALP)活性。结果:辛伐他汀各浓度组均能促进人牙周膜细胞的增殖和分化,其中10-8、10-7、10-6mol/L组与对照组比较,差异有统计学意义(P<0.05),10-7mol/L的辛伐他汀组促进细胞增殖和ALP活性的作用最明显。结论:适宜浓度的辛伐他汀可有效促进人牙周膜细胞的增殖和成骨分化。

Objective： To study the effects of Simvastatin on the proliferation and osteoblastic differentiation of human periodontal lig- ament （PDL） cells. Methods： The fourth passage human PDLs were cultured in conditioned mineralization medium with different concentrations of Simvastatin. Proliferation ability was detected by MTr method. Alkaline phosphatase （ALP） activity was measured with 4-nitrophenyl phosphate hexahydrate （PNPP） assay. Results： Proliferation and osteoblastic differentiation of human PDLs were increased in all treatment groups with different concentrations of Simvastatin（ 10^-9 10^-8, 10^-7, 10^-6 mol/L）. Compared with control group, significant statistical difference was found in 10 ^-8 mol/L, 10 ^-7 mol/L and 10 ^-6 mol/L groups （ P 〈 0.05 ）. The maximum effect was observed at the concentration of 10^-7 mol/L. Conclusion： Optimal concentration of Simvastatin can improve the proliferation and osteoblastic differentiation of human PDLs.