基质金属蛋白酶组织抑制因子-1基因多态性与慢性牙周炎相关性研究

Correlation study of tissue inhibitor of matrix metalloproteinase-1 gene polymorphisms and chronic periodontitis

目的:探讨基质金属蛋白酶组织抑制因子-1(TIMP-1)基因多态性与慢性牙周炎易感性的相关性。方法:从广东汉族人群中收集70例慢性牙周炎(CP)患者及74例牙周健康对照者的颊黏膜拭子,以Chelex-100法提取DNA,采用聚合酶链反应-限制性片段长度多态方法对TIMP-1+372T/C(rs4898)、TIMP-1+533C/T(rs1062849)多态性进行检测,χ2检验分析组间等位基因分布和基因型频率的差异。结果:TIMP-1+372位C、T等位基因在CP组和对照组的分布差异无统计学意义(P=0.897,OR=1.062)。TIMP-1+533C/T多态性未检出。结论:TIMP-1+372T/C多态性与中国广东汉族人慢性牙周炎的患病易感性无关,且该人群中不存在TIMP-1+533C/T多态性。

Objective： To investigate the relationship between TIMP-1 gene polymorphisms and susceptibility of chronic perlodontitis （CP） in Hart Cantonese. Methods： Buccal swabs from 70 CP patients and 74 periodontal healthy controls were collected. DNA was extracted from these buccal swabs by using Chelex- 100. TIMP- 1 ＋ 372T/C （rs4898） ,TIMP- 1 ＋ 533C/T （rs1062849） polymorphisms were tested by polymerase chain reaction -restriction fragment length polymorphism （PCR-RFLP）. Allele distribution and genotypes frequencies in the patients and controls were analyzed. Results ： Frequency variance of allele T and C at site of TIMP- 1 ＋ 372 in pa- tients and controls showed no statistical difference. TIMP-1 ＋ 533C/T polymorphism of TIMP-I hadnI been found in the presem study. Conclusion ： There is no relationship between TIMP- 1 ＋ 372T/C polymorphism and susceptibility of chronic periodontitis, and TIMP- 1 ＋ 533C/T polymorphism doesn＇t exist among Han Cantonese.