龈沟液中神经肽P物质与慢性牙周炎关系临床研究

A clinical study on the relationship between neuropeptide substance P in gingival crevicular fluid and chronic periodontitis

目的:探讨龈沟液中神经肽P物质(SP)与慢性牙周炎发生发展的关系。方法:研究组48例,对照组45例,研究组口内各选1个牙周炎牙位、对照组各选1个健康牙位收集龈沟液样本,采用放射免疫分析技术分别测定2组龈沟液SP含量;记录牙龈指数(GI)、牙周袋探诊深度(PD)、附着丧失(AL)、牙槽骨吸收(ABL)情况,并进行与SP的相关性分析。结果:研究组SP含量显著高于对照组(t=9.92,P<0.01);轻度牙周炎SP含量及牙周临床指标与对照组相比,均有显著差异(P<0.01);轻度牙周炎龈沟液SP含量与GI无显著相关性,但与PD、AL、ABL之间,以及中、重度牙周炎龈沟液SP含量与GI之间,均呈显著性相关(P<0.05);中、重度牙周炎SP含量与PD、AL、ABL之间均呈非常显著性相关(P<0.01)。结论:龈沟液中SP含量与慢性牙周炎的发生发展密切相关,可以反映牙周组织的破坏程度;测定患者龈沟液中SP含量,对于判断病情、指导治疗具有重要意义。

Objective： To study the rclationsbip between occun＇ence and development of chronic periodontitis and neuropeptide sub- stance P （SP） in gingival crevieular fluid. Methods： There were 48 patients in experimental group and 42 patients in the control group. Gingival crevicular fluid samples were collected from the patients＇ intraoral chronic periodontitis inflammatory positive sites and healthy sites. Neuropeptide substance P levels in gingival crevicular fluid from two groups were measured by radioimmunoassay. Gingi- val index, pocket depth, attachment loss, alveolar bone resorption were recorded and their correlations with SP were analyzed. Re- suits： SP levels of the experimental group were significantly higher than those of the control group（ P 〈0.01 ）. There were significant differences（P 〈0.01 ）between SP level and periodontal clinical index in slight periodontitis and the control group. There were signifi- cant correlations（P 〈 0.05 ）between GCF SP content and all PD, AL, ABL but GI in slight periodontitis, and there were significant correlations in between GCF SP content and GI for both moderate periodontitis and severe periodontitis. There were significant correla- tions （ P 〈 0.01 ） between GCF SP content and PD, AL, ABL for both moderate periodontitis and severe periodontitis. Conclusion ： GCF SP level is closely related to occurrence and development of chronic periodontitis. GCF SP level can reflect the degree of the damage of periodoutium. Determination of patients＇ GCF SP level has important significance for the patients＇ condition judgement and treatment guidance.