鸟氨酸脱羧酶抗酶抑制子基因在小鼠发育过程中的作用

Ornithine Decarboxylase Antizyme Inhibitor （Oazin） Induces Mouse Death at Newborn Stage

目的探索鸟氨酸脱羧酶抗酶抑制子基因在小鼠发育过程中的功能。方法用特殊的基因诱捕载体（genetrapping vector）制作了鸟氨酸脱羧酶抗酶抑制子基因敲除小鼠。用Western印迹、RT-PCR、X-gal染色方法分析该基因在突变小鼠体内的表达。PCR法鉴定小鼠不同发育时期的基因型。结果鸟氨酸脱羧酶抗酶抑制子基因在纯合型突变小鼠体内被抑制后，纯合型突变小鼠在出生后死亡。结论鸟氨酸脱羧酶抗酶抑制子基因是致死基因，对小鼠生存有不可缺少的作用。

Objective This study was aimed to identify the function of ornithine decarboxylase antizyme inhibitor （Oazin） gene in mouse development. Methods We have generated Oazin gene inhibited mice by using gene trapping method. The expression pattern of Oazin in the mutant line was analyzed with Western blot, RT-PCR and X-gal staining. Heterozygous mutant mice were mated and all offsprings in different developmental stage were genotyped with PCR. Results The expression of Oazin gene was inhibited in mutant mice. And all homozygous mutant mice were dead at PO stage. Conclusion The results demonstrated that ornithine decarboxylase antizyme inhibitor is a lethal gene and plays a crucial role in mouse surviving.