基因转染骨髓间充质干细胞诱导分化为胰岛素分泌细胞

Differentiation of bone marrow mesenchyaml stem cells into insulin-secreting cells using gene transfection

骨髓间充质干细胞在体外一定条件下分化成为胰岛细胞,既可以解决胰岛移植中供体紧缺的问题,又能够通过自体移植避免免疫排斥,还将避开胚胎干细胞研究所涉及的伦理道德问题,是治疗糖尿病的理想干细胞来源。研究发现可以将胰岛素基因及其他必需的基因直接导入来自糖尿病患者的原代细胞或其他成体干细胞如肝细胞、肠道上皮K细胞、成纤维细胞及肝脏肿瘤细胞等,使其转变为胰岛素分泌细胞即转基因的胰岛素分泌细胞。目前国际上主要的研究方向是如何将胰岛素基因有效地导入靶细胞,并使之呈生理模式表达。

Bone marrow mesenchymal stem cells is a new approach for diabetes treatment, it can transdifferentiate into insulin cells in vitro, that may overcome the shortage of human donor islets, avoid the immunological rejection and moral principle problems which are involved into the research of embryonic stem cells. Studies have shown that insulin gene and its other necessary gene can introduce into primary cells or other somatic stern cells directly in diabetes mellitus patients, such as hepatic cells, intestines epidermis K cells, fibroblast cells and liver tumor cells and so on. Thereby, it can be translated into insulin-secreting cells. At present, the main study direction internationally is how to import insulin gene into target cells effectively, and make it express in a physical model.