L-谷氨酰胺对大鼠次声性脑损伤的防护效应

The protecting effects of L-glutamine on the injury of infrasound to rats' brains

目的:探讨L-谷氨酰胺对16Hz130dB次声暴露下大鼠学习记忆能力、血清S-100β蛋白的影响。方法:将60只雄性SD大鼠随机分为正常对照组、次声组、次声+药物组及药物→次声组,分别将次声组、次声+药物组及药物→次声组暴露于16Hz130dB次声环境中,正常对照组也置于次声舱内,但不给予次声作用。次声+药物组及药物→次声组分别在次声暴露后和次声暴露前饲料中加入药物。上述各组大鼠经7d相应处理后测试其记忆功能、S-100β蛋白水平的变化情况。结果:与正常对照组比较,次声对照组记忆功能明显下降(P<0.01),S-100β蛋白水平有明显升高(P<0.01);次声+药物组、药物→次声组与次声组比较,其学习记忆功能有显著性提高(P<0.05),S-100β蛋白水平明显降低(P<0.05)。结论:16Hz130dB次声可引发大鼠脑损伤,使其记忆功能受损,S-100β蛋白水平升高;L-谷氨酰胺对次声性脑损伤具有防护和治疗的效应。

Objective ：To study the effects of L-glutamine on learning and memory abilities and the contents of S-100β protein in serum of rats exposed to 16Hz 130dB infrasound. Method：Sixty male SD rats were randomly divided into control group, infrasound group,infrasound plus drug-treatment group and drug-prevention plus infrasound group. Learning and memory abilities and the serum concentrations of S-100β protein were measured 7 d after corresponding intervention. Result： In infrasound group compared with control group, rats＇memory abilities went down significantly （P〈O.O1） and serum levels of S-100β protein heightened significantly （P〈0.01）. Compared with those in infrasound group,in drug-treatment group and drug-prevention group rats＇memory abilities raised （P〈 0.05）and the serum levels of S-100β protein lowered （P〈0.05）. Conclusion： Rats＇learning and memory abilities could be damaged by 16Hz 130dB infrasound, which also caused S-100β protein heightening in rats＇serum, while L-glutamine could alleviate the infrasonic injury to rats＇brains.