复方地黄方对帕金森病大鼠神经行为学及氧化应激的影响

Effects of compound rehmannia prescription on the neuroethology and oxidative stress in rats with Parkinson's disease

目的:观察复方地黄方对帕金森病(PD)大鼠神经行为学及氧化应激的影响。方法:采用6-羟基多巴胺(6-OHDA)注射于脑右侧黑质造成偏侧PD模型。模型组用复方地黄方(4.14g/ml,2ml/d)中药进行治疗,此外设立了正常对照组、假手术组。观察PD大鼠神经行为学、氧化应激的变化及复方地黄方的作用。结果:PD模型组进行复方地黄方治疗后有明显的神经行为学改变,谷胱甘肽过氧化物酶(GSH-Px)、谷胱甘肽(GSH)、超氧化物歧化酶(SOD)含量均明显降低,活性氧与丙二醛(MDA)含量升高。结论:复方地黄方能明显改善PD模型大鼠的旋转行为,提高其抗氧化能力和清除自由基的能力。

Objective： To observe the effects of compound rehmannia prescription on neuroethology and oxidative stress in rats with Parkinson＇s disease （PD）. Method： Model of hemilateral PD rat was established with injection of 6-hydroxydopamine （6-OHDA） into the substantia nigra of right side brain. The rats in PD model group were treated with compound rehmannia prescription （4.14g/ml,2ml/d）;in addition, a normal control group and a sham-operated group were established. The changes of neuroethology and oxidative stress in PD rats after treatment with compound rehmannia prescription were observed. Result：The rats in PD model group after treatment with compound rehmannia appeared obvious changes of neuroethology,the contents of glutathione-peroxidase （GSH-Px）,glutathione （GSH）,superoxide dismutase （SOD） reduced significantly, and the contents of reactive oxygen species （ROS） and malondialdehyde （MDA） elevated significantly.Conclusion： Compound rehmannia prescription can significantly improve the spinning behavior of PD model rats,and elevate it＇s antioxidant ability and scavenging free radicals ability.