黄芪总苷对一次性力竭游泳大鼠脑组织三磷酸腺苷酶活性的影响

Effects of astragalosides on ATPase activity of brain in rats after one-off exhaustive swimming

目的:探讨黄芪总苷对一次性力竭游泳大鼠脑组织三磷酸腺苷(ATP)酶活性的影响。方法:24只SD大鼠随机均分为3组(对照组、模型组、黄芪总苷组),对照组大鼠常规饲养3周后处死取材,黄芪总苷组预防性给药3d,模型组和黄芪总苷组在一次力竭性游泳运动后取样,观察大鼠脑组织ATP酶活性的变化。结果:力竭游泳大鼠脑组织Na+K+-ATPase、Mg2+-ATPase、Ca2+-ATPase和Ca2+Mg2+-ATPase活力均较正常对照组有明显降低(P<0.05),黄芪总苷能提高力竭运动大鼠脑组织的ATP酶活性,虽均尚不能恢复到正常水平,但Mg2+-ATPase、Ca2+Mg2+-ATPase的活力已与模型组有显著性差异(P<0.05)。结论:黄芪总苷预防给药具有保护力竭运动大鼠脑组织的作用。

Objective： To observe the effects of astragalosides on adenosine triphosphatase （ATPase） activity of brain in rats after one-off exhaustive swimming. Method： Twenty-four SD rats were randomly divided into 3 groups （normal control group, model group, astragalosides group）. The rats in normal control group were sacrificed after normal raising; astragalosides were administrated to the rats prophylactically in astragalosides group for 3d; the rats in model group and astragalosides group were sacrificed after one -off exhaustive swimming. Then the activity of ATPase were observed in the brain of all rats. Result： The activity of Na^＋K^＋-ATPase、Mg^2^＋-ATPase、Ca^2^＋-ATPase and Ca^2^＋Mg^2^＋-ATPase in the brain of exhaustive swimming rats reduced significantly than that in normal control group （P〈0.05）. Administration of astragalosides could markedly elevate the activity of ATPase in the brain of exhaustive swimming rats. Although the activity of ATPase of rats in astragalosides group couldn＇t reach normal level, the activities of Mg^2^＋-ATPase、Ca^2^＋Mg^2^＋-ATPase in astragalosides group were distinctly different from model group （P〈 0.05）. Conclusion： Astragalosides could provide protective effect on brain in exhaustive exercises rats.