摘要
采用数据库搜索及ISSR-抑制PCR法开发香菇微卫星标记。由数据库搜索法开发出21对引物,11对有多态性,各位点平均产生3.3个等位基因;通过ISSR-抑制PCR法开发出8对引物,5对具多态性,各位点平均产生3个等位基因。结果表明,在香菇SSR开发中,两种方法均是行之有效的。
Two methods, database search and ISSR-suppression PCR, were used to isolate microsatellite markers from Lentinula edodes. Twenty-one primer pairs were designed by database search, and eleven of them showed polymorphism. The number of alleles produced by these eleven markers was 3.3 per locus in average. Using method of ISSR-suppression PCR, eight primer pairs were developed and five of them had polymorphism. There were 3 alleles per locus in average produced by these five markers. The results showed that two methods were effective and applicable to develop SSR from L. edodes.
出处
《菌物学报》
CAS
CSCD
北大核心
2009年第4期548-552,共5页
Mycosystema
基金
公益性行业(农业)科研专项"食用菌菌种质量评价与菌种信息系统研究与建立"(No.nyhyzx07-008)
农学教学实验用微生物菌种资源标准化整理
整合及共享试点(No.2005DKA21208-6)
