斜生褐孔菌多糖组分的纯化及其生物活性研究

Purification of polysaccharide of Phaeoporus obliquus and the bioactivity of purified fractions

对斜生褐孔菌子实体和菌丝体的多糖含量进行了测定,二者多糖含量分别为2.63%和2.12%。运用DEAE Sephacel和Sephadex G-200柱层析技术从子实体中纯化出B1、B2、B31和B32四种多糖组分,分别占子实体多糖总量的50.20%、32.25%、4.48%和6.61%;从菌丝体中纯化出M1、M2和M3三种多糖组分,分别占菌丝体多糖总量的48.23%、31.85%和10.91%。生物活性试验结果显示:七种纯化多糖组分的抗氧化性由强到弱依次为B1>M1>M2>B2>M3>B32>B31;B2和M2具有在体外抑制人体白血病细胞株K-562和肝癌SMMC772l细胞株生长的作用;B31、B32和M3具有降低四氧嘧啶糖尿病小鼠血糖的作用,而且对健康小鼠的血糖值并无影响。

The content of polysaccharide of fruiting body and mycelia of Phaeoporus obliquus were determined and their values were 2.63% and 2.12% respectively. The methods of DEAE Sephacel column chromatography and Sephades G-200 column chromatography were employed to purify the raw polysaccharide of fruiting body and mycelia. The results showed that there were four kinds of fractions in raw polysaccharide of fruiting body, and they were marked as B 1, B2, B31 and B32 and their contents were 50.20%, 32.25%, 4.48% and 6.61% respectively; fractions M1, M2 and M3 were purified from raw polysaceharide of myeelia and their contents were 48.23%, 31.85% and 10.91% respectively. Bioactivity of purified fractions were studied and the results showed that antioxidative activities were in sequence of B 1 〉 M 1 〉 M2 〉 B2 〉 M3 〉 B32 〉 B31. B2 and M2 could inhibit the proliferation of leukemia K-562 cells and lung cancer SMMC7721 cells in vitro; B31, B32 and M3 could reduce the blood sugar of mouse with alloxan diabetes and don＇t affect the blood sugar of healthy mouse.