杜仲内生球毛壳菌的抗氧化活性研究

Antioxidant activity of endophytic Chaetomium globosum from Eucommia ulmoides

本文研究了分离自杜仲叶片的内生真菌球毛壳菌菌株No.173发酵液提取物的抗氧化活性,采用铁氰化钾还原力测定法、β-胡萝卜素/亚油酸模型和光照核黄素体系评价了发酵液提取物的抗氧化作用,并采用Folin-Ciocalteu法测定杜仲内生球毛壳菌发酵液提取物总多酚含量。结果表明,其抗氧化能力与Vc基本相当;清除超氧阴离子的能力优于芦丁;多酚含量为255.53±1.38mg/g,是其主要的抗氧化活性成分。

Antioxidant activity of fermentation broth extracts of Chaetomium globosum strain No. 173 isolated from leaves of Eucommia ulmoides was studied. The reducing power determination, the bleaching of β-carotene suboleic acid model and the riboflavine photosensitization system were applied to evaluate the antioxidant effects of the extracts. Total phenolic content in the extracts was estimated by using the Folin-Cincalteu colorimetric method. The results showed that the antioxidant capacity of the extracts was equivalent to Vc, and the capacity scavenging O2^-＊ was better than rutin. The phenolic content of the extracts was 255.53±1.38mg/g （gallic acid equivalent）.