不同中药对奶牛乳腺上皮细胞抗氧化能力的影响

Effect of Herbs on Antioxidative Activities of Bovine Mammary Gland Epithelial Cells

为研究中药对奶牛乳腺抗氧化能力的影响,选取7种中草药(王不留行、黄芪、漏芦、木通、通草、蒲公英、甲珠)测定其对体外培养的奶牛乳腺上皮细胞抗氧化能力的影响。测定了奶牛乳腺上皮细胞中的超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)活性、丙二醛(MDA)质量分数、一氧化氮(NO)含量和一氧化氮合酶(NOS)活性。结果表明中药组奶牛乳腺上皮细胞SOD、GSH-Px和CAT活性高于或显著高于正常对照组(P>0.05,P<0.05,P<0.01),MDA、NO、NOS质量分数低于或显著低于正常对照组(P>0.05,P<0.05,P<0.01)。中药能减少脂质过氧化产物MDA的生成,同时提高奶牛抗氧化酶活性,增强了奶牛的抗氧化能力。

Seven Chinese herbs were selected to study their effects on antioxidative activity of bovine mammary gland, they are cowherb seed, radix astragali,dandelion,akebia stem, medulla tetrapanacis, radix rhapontici and pangolin scales. The activity of SOD, GSH-Px, CAT and the content of MDA, NO, NOS in bovine mammary gland epithelial cells were detected. The results showed that the activities of SOD, GSH-Px and CAT in bovine mammary gland epithelial cells were higher or significantly higher in herbs groups compared to the control （P〉0.05, P〈0.05, P〈0.01）. Simultaneously, the content of MDA in bovine mammary gland epithelial cells from herbs group was lower or significantly lower than the control （P〉0.05, P〈0.05, P〈0.01）. Herbs could reduce MDA,NO,NOS and enhance the antioxidase activity of bovine mammary gland. It strengthen antioxidative activity of bovine.